An electrochemical deamidated gliadin antibody immunosensor for celiac disease clinical diagnosis
نویسندگان
چکیده
منابع مشابه
An electrochemical deamidated gliadin antibody immunosensor for celiac disease clinical diagnosis.
The first electrochemical immunosensor (EI) for the detection of antibodies against deamidated gliadin peptides (DGP) is described here. A disposable nanohybrid screen-printed carbon electrode modified with DGP was employed as the transducer's sensing surface. Real serum samples were successfully assayed and the results were corroborated with an ELISA kit. The presented EI is a promising analyt...
متن کاملElectrochemical immunosensor for the diagnosis of celiac disease.
A novel electrochemical immunosensing strategy for the detection of antibodies to tissue transglutaminase (tTG) in human serum is presented. The proposed immunosensor consists of the immobilization by physical adsorption of tTG from guinea pig liver on graphite-epoxy composite (GEC) electrodes. After the reaction with the human serum (containing the specific antibodies in the case of celiac dis...
متن کاملIgG antibodies against deamidated gliadin peptides for diagnosis of celiac disease in patients with IgA deficiency.
BACKGROUND Assays for IgG antibodies against deamidated gliadin (IgG-anti-dGli) are comparable in performance with tests detecting IgA antibodies against tissue transglutaminase (IgA-anti-tTG) in diagnosing celiac disease (CD). IgA-anti-tTG are absent in IgA deficiency, a condition often associated with CD. In IgA deficiency, IgG-anti-tTG, which have a lower overall diagnostic accuracy, are rou...
متن کاملCeliac disease: antibody recognition against native and selectively deamidated gliadin peptides.
BACKGROUND Selective deamidation of glutamine residues by tissue transglutaminase (tTG) turns gliadin peptides into stronger activators of T cells from celiac disease (CD) patients. We examined the possibility that these modified peptides could be more specific epitopes for circulating antibodies than are native peptides. METHODS Two native synthetic peptides and their respective modified seq...
متن کاملAntibodies against synthetic deamidated gliadin peptides for celiac disease diagnosis and follow-up in children.
BACKGROUND AGA IgA II and AGA IgG II have recently been suggested as reliable tools for celiac disease (CD) diagnosis. We compared their utility for diagnosis and monitoring CD in children with that of tTG IgA, an established CD marker. METHODS We studied a cohort of 161 CD and 129 control children in whom CD was histologically confirmed or ruled out. We followed 37 children with CD on a glut...
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ژورنال
عنوان ژورنال: The Analyst
سال: 2013
ISSN: 0003-2654,1364-5528
DOI: 10.1039/c3an36728b